Molecular Detection of Foot and Mouth Disease Virus (FMDV) from 2017 Outbreaks in Punjab by RT-PCR and RT-LAMP Assays
نویسندگان
چکیده
منابع مشابه
Pan-serotypic detection of foot-and-mouth disease virus by RT linear-after-the-exponential PCR.
A reverse transcription Linear-After-The-Exponential polymerase chain reaction (RT LATE-PCR) assay was evaluated for detection of foot-and-mouth disease virus (FMDV). This pan-serotypic assay targets highly conserved sequences within the 3D (RNA polymerase) region of the FMDV genome, and uses end-point hybridisation analysis of a single mismatch-tolerant low temperature probe to confirm the ide...
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Successful disease management requires a rapid and sensitive diagnosis method that can recognize early infection even before the manifestation of its clinical signs. The only available field diagnostic tests for foot-and-mouth disease (FMD) are lateral flow devices, commonly known as chromatographic strips. Low sensitivity and inability to detect FMD virus (FMDV) at the serotype level are limit...
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Rapid antigen and antibody, serological tests, and RT ‑ PCR-based molecular methods are widely used for the detection of microorganisms worldwide. Objectives: This study aimed by Isothermal nucleic acid amplification techniques to detect the covid-19. Methods: In this study, 200 samples of nasopharynx and oropharynx were collected from Jamaran Heart Hospital in Tehran. Covid -19 was exami...
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متن کاملDiagnosis of Foot-and-Mouth disease virus by automated RT-PCR
Automated 5' nuclease probe-based (TaqMan) reverse transcription polymerase chain reaction (RT-PCR) procedures using a MagNA Pure LC were evaluated for foot-and-mouth (FMD) virus diagnosis during the United Kingdom (UK) 2001 epidemic. Epithelial suspensions (ES), serum or whole blood, milk and oesophageal-pharyngeal fluid (“probang”) samples submitted to the OIE/FAO World Reference Laboratory f...
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ژورنال
عنوان ژورنال: Journal of Veterinary Science & Technology
سال: 2018
ISSN: 2157-7579
DOI: 10.4172/2157-7579.1000508